A Novel Designed Chitosan Based Hydrogel Which is Capable of Consecutively Controlled Release of TGF-Beta 1 and BMP-7

摘要: Objective: Both Bone Morphogenetic Protein 7 (BMP-7) and Transforming Growth Factor Beta 1 (TGF- β1) have stimulating effects on chondrocyte proliferation. These growth factors act at different time points of the biological repair process. We aimed to design a hydrogel, which would enable consecutive controlled release factors. Material Methods: de - signed chitosan particle impregnated Poly (vinylalcohol) (PVA)-borax hydrogel (CPBH). CPBH allowed BMP-7 TGF- β1 points. Hydrogel structure was analyzed by Scanning Electron Microscope (SEM). The kinetics Factors (GFs) were determined with enzyme-linked immunosorbent assay (ELISA) UV-spectrophotometer. Chondrocyte viability toxicity tested through CellTiter 96 ® Non-Radioactive Cell Prolif eration (MTS) ELISA. Results: designed showed high swelling mucoadhesion characteristics under acidic conditions. released first rapidly con secutively slowly. It also protein/peptide based drugs for 21 days without altering their bioactive properties. At end days, 82.62% 98.34% consecutively released. difference between groups significant (for β1, p<0.05 BMP-7, p<0.001). Conclusion: slow has been shown be beneficial cartilage regeneration. As it is not cytotoxic, we suggest that this might used in medical pharmaceutical applications areas.