Identification of anther-specific genes in a cruciferous model plant, Arabidopsis thaliana , by using a combination of Arabidopsis macroarray and mRNA derived from Brassica oleracea
作者： Masayuki Amagai , Tohru Ariizumi , Makoto Endo , Katsunori Hatakeyama , Chikara Kuwata
DOI: 10.1007/S00497-002-0159-Z
关键词:
摘要: Arabidopsis thaliana, a member of the Brassicaceae, is model plant whose genome was first higher to be sequenced. Because small size flowers, it difficult dissect and separate reproductive organs (anthers pistils) at different developmental stages in A. thaliana. In order perform genome-wide identification anther-specific genes an cDNA macroarray hybridized derived from anthers pistils another crucifer, Brassica oleracea. After scanning signal intensity for each clone, cluster analysis, 52 were identified. These clones contained several that have already been characterized, as well novel genes. RT-PCR analysis with mRNA thaliana B. oleracea, expression pattern one-third similar determined by macroarray. This system heterologous hybridization (Arabidopsis vs tissue-specific mRNA) should applicable other species their close relatives.
springer.com
sci-hub.se 参考文章(47)
Dawid Ib, Taira M, Toyama R, LIM domain proteins. Comptes Rendus De L Academie Des Sciences Serie Iii-sciences De La Vie-life Sciences. ,vol. 318, pp. 295- 306 ,(1995)
Agnès Fourgoux-Nicol, Jan Drouaud, Najat Haouazine, Georges Pelletier, Philippe Guerche, Isolation of rapeseed genes expressed early and specifically during development of the male gametophyte. Plant Molecular Biology. ,vol. 40, pp. 857- 872 ,(1999) , 10.1023/A:1006282507095
Hyun Uk Kim, Tae Young Chung, Characterization of three anther-specific genes isolated from Chinese cabbage. Plant Molecular Biology. ,vol. 33, pp. 193- 198 ,(1997) , 10.1023/A:1005790832221
M F Manolson, B F Ouellette, M Filion, R J Poole, cDNA sequence and homologies of the “57-kDa” nucleotide-binding subunit of the vacuolar ATPase from Arabidopsis. Journal of Biological Chemistry. ,vol. 263, pp. 17987- 17994 ,(1988) , 10.1016/S0021-9258(19)81313-3
Peter Rubinelli, Yi Hu, Hong Ma, Identification, sequence analysis and expression studies of novel anther-specific genes of Arabidopsis thaliana Plant Molecular Biology. ,vol. 37, pp. 607- 619 ,(1998) , 10.1023/A:1005964431302
Kazuhiko Nishitani, The Role of Endoxyloglucan Transferase in the Organization of Plant Cell Walls International Review of Cytology-a Survey of Cell Biology. ,vol. 173, pp. 157- 206 ,(1997) , 10.1016/S0074-7696(08)62477-8
Zoe A Wilson, Shaun M Morroll, Janet Dawson, Ranjan Swarup, Paddy J Tighe, None, The Arabidopsis MALE STERILITY1 (MS1) gene is a transcriptional regulator of male gametogenesis, with homology to the PHD-finger family of transcription factors The Plant Journal. ,vol. 28, pp. 27- 39 ,(2001) , 10.1046/J.1365-313X.2001.01125.X
Masao Watanabe, Hideyuki Shiozawa, Akira Isogai, Akinori Suzuki, Takuji Takeuchi, Kokichi Hinata, Existence of S-Glycoprotein-Like Proteins in Anthers of Self-Incompatible Species of Brassica Plant and Cell Physiology. ,vol. 32, pp. 1039- 1047 ,(1991) , 10.1093/OXFORDJOURNALS.PCP.A078167
R. L. Allen, D. M. Lonsdale, Sequence analysis of three members of the maize polygalacturonase gene family expressed during pollen development. Plant Molecular Biology. ,vol. 20, pp. 343- 345 ,(1992) , 10.1007/BF00014505
Makoto Endo, Hitoshi Matsubara, Takao Kokubun, Hiromi Masuko, Yoshihito Takahata, Tohru Tsuchiya, Hiroo Fukuda, Taku Demura, Masao Watanabe, The advantages of cDNA microarray as an effective tool for identification of reproductive organ-specific genes in a model legume, Lotus japonicus. FEBS Letters. ,vol. 514, pp. 229- 237 ,(2002) , 10.1016/S0014-5793(02)02371-2