Gold nanorod-based fluorometric ELISA for the sensitive detection of a cancer biomarker

摘要: The combinatorial use of an enzyme-linked immunosorbent assay (ELISA) and nanoparticles (i.e. nanoELISA) facilitates the improved detection biomarkers. In present study, we have developed a gold nanorod-based fluorometric immunoassay (nanoELIFA) for non-invasive CA125 (an ovarian cancer biomarker) using fluorogenic substrate, 3-p-hydroxyphenylpropionic acid (HPPA). NanoELIFA showed sensitivity (detection limit 0.066 U mL−1) over classical ELIFA limit: 0.23 due to integration nanorods, which act as signal enhancer with additional advantage fluorescence-based sensitivity. analyses was tested in range 0–400 mL−1. Furthermore, reproducibility selectivity nanoELIFA were analyzed indicative good performance system. This method initially spiked simulated blood plasma cell culture media, finally, (as such spiked) supernatant media from vitro samples. recovery percentages indicated all these cases highlighted applicability real sample analysis.