Extraction, Antioxidant Capacity, 5-Lipoxygenase Inhibition, and Phytochemical Composition of Propolis from Eastern Canada.
作者： Mariama Sambou , Jacques Jean-François , Fanta J. Ndongou Moutombi , Jérémie A. Doiron , Mathieu P.A. Hébert
DOI: 10.3390/MOLECULES25102397
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摘要: Soxhlet (SE), microwave-assisted (MAE) and ultrasound-assisted (UAE) extraction were compared using ten solvents for their efficiency to extract phenolic flavonoid antioxidants from Eastern Canada propolis. Extracts total (TPC) (TFC) content, radical scavenging activities. Anti-inflammatory activity through inhibition of 5-lipoxygenase (5-LO) products biosynthesis in HEK293 cells was also evaluated. The results showed that SE extracts polar had the highest TPC TFC. obtained with ethanol, methanol acetone effective free scavengers, 5-LO similar zileuton. UAE an method since comparable those MAE while being done at room temperature. With UAE, less much more such as or ethanol. Reversed-phase liquid chromatography tandem mass spectrometry confirmed presence 21 natural compounds propolis based on comparison intact mass, chromatographic retention time fragmentation patterns derived commercial analytical standards. current study is first its kind concurrently investigate solvent polarity well techniques
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