作者: Yuichi Murayama , Miyako Yoshioka , Hiroyuki Okada , Eri Takata , Kentaro Masujin
DOI: 10.1371/JOURNAL.PONE.0144761
关键词:
摘要: The global outbreak of bovine spongiform encephalopathy (BSE) has been attributed to the recycling contaminated meat and bone meals (MBMs) as feed supplements. use MBMs prohibited in many countries; however, development a method for inactivating BSE prions could enable efficient safe these products an organic resource. Subcritical water (SCW), which is heated under pressure maintain liquid state at temperatures below critical temperature (374°C), exhibits strong hydrolytic activity against compounds. In this study, we examined residual vitro seeding protease-resistant prion protein (PrPSc) infectivity after SCW treatments. Spinal cord homogenates prepared from BSE-infected cows were treated with 230-280°C 5-7.5 min used intracerebrally inoculate transgenic mice overexpressing protein. Serial misfolding cyclic amplification (sPMCA) analysis detected no PrPSc SCW-treated homogenates, samples survived more than 700 days without any signs disease. However, sPMCA analyses accumulation brains all inoculated mice. Furthermore, secondary passage mice, brain derived western blotting (WB)-positive primary mouse, died average 240 days, similar untreated spinal homogenates. vacuolation typically observed confirmed suggesting that maintained their treatment. One late-onset case, well asymptomatic but sPMCA-positive cases, also recognized WB-negative These results indicated SCW-mediated hydrolysis was insufficient eliminate conditions tested.