Subcritical Water Hydrolysis Effectively Reduces the In Vitro Seeding Activity of PrPSc but Fails to Inactivate the Infectivity of Bovine Spongiform Encephalopathy Prions

作者: Yuichi Murayama , Miyako Yoshioka , Hiroyuki Okada , Eri Takata , Kentaro Masujin

DOI: 10.1371/JOURNAL.PONE.0144761

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摘要: The global outbreak of bovine spongiform encephalopathy (BSE) has been attributed to the recycling contaminated meat and bone meals (MBMs) as feed supplements. use MBMs prohibited in many countries; however, development a method for inactivating BSE prions could enable efficient safe these products an organic resource. Subcritical water (SCW), which is heated under pressure maintain liquid state at temperatures below critical temperature (374°C), exhibits strong hydrolytic activity against compounds. In this study, we examined residual vitro seeding protease-resistant prion protein (PrPSc) infectivity after SCW treatments. Spinal cord homogenates prepared from BSE-infected cows were treated with 230-280°C 5-7.5 min used intracerebrally inoculate transgenic mice overexpressing protein. Serial misfolding cyclic amplification (sPMCA) analysis detected no PrPSc SCW-treated homogenates, samples survived more than 700 days without any signs disease. However, sPMCA analyses accumulation brains all inoculated mice. Furthermore, secondary passage mice, brain derived western blotting (WB)-positive primary mouse, died average 240 days, similar untreated spinal homogenates. vacuolation typically observed confirmed suggesting that maintained their treatment. One late-onset case, well asymptomatic but sPMCA-positive cases, also recognized WB-negative These results indicated SCW-mediated hydrolysis was insufficient eliminate conditions tested.

参考文章(40)
Miyako Yoshioka, Morikazu Imamura, Hiroyuki Okada, Noriko Shimozaki, Yuichi Murayama, Takashi Yokoyama, Shirou Mohri, Sc237 hamster PrPSc and Sc237-derived mouse PrPSc generated by interspecies in vitro amplification exhibit distinct pathological and biochemical properties in tga20 transgenic mice Microbiology and Immunology. ,vol. 55, pp. 331- 340 ,(2011) , 10.1111/J.1348-0421.2011.00328.X
Richard Race, Bruce Chesebro, Scrapie infectivity found in resistant species Nature. ,vol. 392, pp. 770- 770 ,(1998) , 10.1038/33834
Prions: a challenge for science, medicine and public health system. Prions: a challenge for science, medicine and public health system.. ,(2000) , 10.1159/ISBN.978-3-318-00622-3
D.M. Taylor, Resistance of transmissible spongiform encephalopathy agents to decontamination. Contributions to microbiology. ,vol. 11, pp. 136- 145 ,(2004) , 10.1159/000077054
H. Yoshida, M. Terashima, Y. Takahashi, Production of organic acids and amino acids from fish meat by sub-critical water hydrolysis Biotechnology Progress. ,vol. 15, pp. 1090- 1094 ,(1999) , 10.1021/BP9900920
Yuichi Murayama, Miyako Yoshioka, Hiroko Horii, Masuhiro Takata, Takashi Yokoyama, Takashi Sudo, Koichi Sato, Morikazu Shinagawa, Shirou Mohri, Protein misfolding cyclic amplification as a rapid test for assessment of prion inactivation. Biochemical and Biophysical Research Communications. ,vol. 348, pp. 758- 762 ,(2006) , 10.1016/J.BBRC.2006.07.130
Hiroko K. Hayashi, Takashi Yokoyama, Masuhiro Takata, Yoshifumi Iwamaru, Morikazu Imamura, Yuko K. Ushiki, Morikazu Shinagawa, The N-terminal cleavage site of PrPSc from BSE differs from that of PrPSc from scrapie Biochemical and Biophysical Research Communications. ,vol. 328, pp. 1024- 1027 ,(2005) , 10.1016/J.BBRC.2005.01.065
Xiaoxia Liang, Qiaojia Fan, Application of Sub-Critical Water Extraction in Pharmaceutical Industry Journal of Materials Science and Chemical Engineering. ,vol. 01, pp. 1- 6 ,(2013) , 10.4236/MSCE.2013.15001