Methyl Sartortuoate Inhibits Colon Cancer Cell Growth by Inducing Apoptosis and G2/M-Phase Arrest.

摘要: The potential anti-neoplastic activity of terpenoids is continued interest. In this study, we investigate whether methyl sartortuoate, a terpenoid isolated from soft coral, induced cell cycle arrest and apoptosis in human colon cancer line. Culture studies found that sartortuoate inhibited (LoVo RKO) growth caused apoptotic death concentration- time-dependent manner, by activation caspase-8, caspase-9, caspase-3, p53 Bax, inactivation B-cell lymphoma 2 (Bcl-2) regulating proteins. Methyl treatment led to reduced expression cdc2 up-regulated p21 p53, suggesting G2-M through modulation p53/p21/cdc2 pathways. also phospho-JNK phospho-p38 levels. This resulted at the phase LoVo RKO cells. Treatment with JNK inhibitor SP600125 p38 MAPK SB203580 prevented sartortuoate-induced Moreover, neoplasm NOD-SCID nude mice inoculated Taken together, these findings suggest capable leading -9, -3, increasing Bax/Bcl-2 ratio MAPK-dependent results Thus, may be promising anticancer candidate.