Effect of diphtheria toxin on protein synthesis: inactivation of one of the transfer factors.
作者： R.J. Collier
DOI: 10.1016/0022-2836(67)90280-X
关键词:
摘要: Abstract The mechanism by which diphtheria toxin inhibits amino acid transfer from aminoacyl-sRNA to ribosomes has been studied in the reticulocyte cell-free system. In presence of required cofactor NAD, found inactivate one supernatant factors 65 85%; complementary factor is not affected. Removal NAD gel filtration does reverse inactivation. Aminoacyl-sRNA and treated with plus are fully active after removal NAD. Toxin break down polysomes single release nascent polypeptide chains ribosomes.
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