Host genetic and environmental effects on mouse intestinal microbiota.

摘要: The mammalian gut harbors complex and variable microbial communities, across both host phylogenetic space conspecific individuals. A synergy of genetic environmental factors shape these communities account for their variability, but individual contributions the selective pressures involved are still not well understood. We employed barcoded pyrosequencing V1-2 V4 regions bacterial small subunit ribosomal RNA genes to characterize effects genetics environment on cecum assemblages in 10 genetically distinct, inbred mouse strains. Eight strains foundation Collaborative Cross (CC), a panel mice derived from diverse set founder strains, designed specifically trait analysis. Diversity microbiota was characterized by complementing distance-based, sequence-clustering approaches. Significant correlations were found between microbiota, reflected distinct communities. Cohabitation litter had reduced, although detectable effect, response varied strain. identified phylotypes that appear be discriminative strain-specific each line used. different revealed an interaction shaping consortia, which became more similar retained strain specificity. This study provides baseline analysis intestinal eight CC progenitor will linked integrated genotype, phenotype research resulting panel.