Intron-dependent recruitment of pre-mRNA splicing factors to sites of transcription.

摘要: We have examined the nuclear localization of transiently and stably expressed nascent RNA transcripts containing or lacking introns in order to determine if spatial association pre-mRNA splicing factors nuclei is random functionally significant. Our findings show that between nucleus intron-dependent when RNAs are either expressed. Furthermore, our data indicate recruited transcription sites. The presence both pre-and mRNA at these locations suggest occurs sites transcription. In addition, electron microscopic examination highly active has revealed a granular appearance which closely resembles, but different from, interchromatin granule clusters. demonstrate organized dynamic with regard functions splicing.