Chebulic Acid Prevents Methylglyoxal-Induced Mitochondrial Dysfunction in INS-1 Pancreatic β-Cells
作者： Hyun-jung Yoo , Chung-Oui Hong , Sang Keun Ha , Kwang-Won Lee
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摘要: To investigate the anti-diabetic properties of chebulic acid (CA) associated with prevention methyl glyoxal (MG)-induced mitochondrial dysfunction in INS-1 pancreatic β-cells, cells were pre-treated CA (0.5, 1.0, and 2.0 μM) for 48 h then treated 2 mM MG 8 h. The effects on evaluated using following: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; glyoxalase 1 (Glo-1) expression via Western blot enzyme activity assays; Nrf-2, nuclear factor erythroid 2-related protein reactive oxygen species (ROS) production mRNA related components (UCP2, uncoupling 2; VDAC1, voltage-dependent anion-selective channel-1; cyt c, cytochrome c quantitative reverse transcriptase-PCR; membrane potential (MMP); adenosine triphosphate (ATP) synthesis; glucose-stimulated insulin secretion (GSIS) assay. viability was maintained upon pre-treating before exposure to MG. upregulated Glo-1 prevented MG-induced ROS production. Mitochondrial alleviated by pretreatment; this occurred downregulation UCP2, increase MMP ATP synthesis. Further, pre-treatment promoted recovery from decrease GSIS. These results indicated that could be employed as a therapeutic agent diabetes due its ability prevent development sensitivity oxidative stress-induced β-cells.
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