The Biosynthesis of Hyaluronic Acid by Streptococcus

摘要: Abstract The mechanism of hyaluronic acid chain growth in a particulate enzyme preparation obtained from Group A Streptococcus has been examined and additional characteristics the detailed. Hyaluronic elongation occurs by transfer monosaccharide units uridine nucleoside diphosphate derivatives to nonreducing ends endogenous oligosaccharide. This oligosaccharide which appeared be chemically identical with was released either hydrolysis or digestion streptococcal hyaluronidase. only detectable nucleotide product synthesis UDP, not inhibited bacitracin. There no evidence for participation lipid-extractable intermediates during formation. pH optimum polysaccharide formation is 7.1. exhibits an absolute dependence upon divalent metal ion maximally activated 10 mm Mg++ 1 Mn++. Michaelis constants UDP-GlcUA UDP-GlcNAc are 5 x 10-5 m 10-4 m, respectively. inactivated treatment 4% 1-butanol. Addition UDP-GlcUA, UDP-GlcNAc, other sugars prior butanol prevented inactivation. Fifty per cent stabilization occurred at 7 UDP.