Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1

摘要: UW-1 produced 17–24 g L−1 rhamnolipid in vegetable oil-containing media shake flask cultures 13 days. In time course studies of growth and production a salts medium containing 6% canola oil, total bacterial count reached 2.6 × 1010 CFU ml−1 after 48 h maximum yield 24.3 was obtained 9 Rhamnolipid components were purified separated by chloroform-methanol extraction TLC chromatography. The major characterised as L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate nuclear magnetic resonance mass spectrometry. preparatively silica gel column recovered monorhamnosyl fraction contained no dirhamnosyl moiety while the 5% when analyzed HPLC. ratio mono- to P. aeruginosa determined HPLC be 4 : 1 weight. Purified had same CMC value 40 μg decreased surface tension water 27.7 30.4 dynes cm−1, respectively.