Early detection of neonatal group B streptococcus sepsis and the possible diagnostic utility of IL-6, IL-8, and CD11b in a human umbilical cord blood in vitro model.

摘要: BACKGROUND Group B streptococcus (GBS) infection remains a major cause of neonatal morbidity and mortality, GBS III is the predominant strain in early-onset sepsis. To avoid both over- undertreatment infants with nonspecific signs infection, early diagnostic tools are warranted. The aim this study was to identify biomarkers high sensitivity specificity an stage infection. A secondary assess utility human umbilical cord blood (HUCB) model system METHODS Umbilical samples from 20 healthy term pregnancies were stimulated for 2 hours isolate patient commercially available Ia strain. Nonstimulated served as controls. Leukocyte surface markers (CD11b, CD64, toll-like receptor [TLR] 2, TLR4, TLR6) analyzed by flow cytometry soluble enzyme-linked immunosorbent assay (interleukin [IL]-6 -8; interferon-γ-inducing protein [IP]-10; S100b). area under receiver operating characteristic curve (AUC) calculated markers. RESULTS gave highest responses AUC values all biomarkers. Only IL-6 IL-8 displayed approaching 0.8 serotypes (P 5,292 pg/mL had 1.00. >197 0.95 stimulation. CD11b on granulocytes monocytes leukocyte marker serotypes. CONCLUSION In agreement previous studies, IL-6, IL-8, potentially could be useful diagnosing stage. Our HUCB sepsis may evaluating neonates risk factors might even used purposes, but requires further study.