Membrane-wall attachments in plasmolysed plant cells.

摘要: Field emission scanning electron microscopy of plasmolysed Tradescantia virginiana leaf epidermal cells gave novel insights into the three-dimensional architecture Hechtian strands, reticulum, and inner surface cell wall without need for extraction. At high magnification, we observed fibres that pin plasma membrane to after plasmolysis. Treatment with cellulase caused these connecting be lost pinned out reticulum disintegrate vesicles diameters 100-250 nm. This suggests may cellulose. After 4 h plasmolysis, a fibrous meshwork labelled anti-callose antibodies was within space between protoplast by field microscopy. Interestingly, macerase-pectinase treatment resulted in loss this meshwork, suggesting it stabilised pectins. We suggest cellulose microfibrils extending from strands entwining matrix act as anchors moves away during