Adhesion to E-selectin promotes growth inhibition and apoptosis of human and murine hematopoietic progenitor cells independent of PSGL-1

摘要: Although both P- and E-selectin are constitutively expressed on bone marrow endothelial cells, their role in the regulation of hematopoiesis has only recently been investigated. We have previously shown that P-selectin glycoprotein ligand-l (PSGL-1/CD162) is by primitive human CD34 mediates adhesion to P-selectin, and, more importantly, inhibits proliferation. now demonstrate proliferation cells isolated either from umbilical cord blood, adult mobilized or steady-state marrow. Furthermore, a subpopulation, which does not contain most hematopoietic progenitor undergoes apoptosis following E-selectin–mediated adhesion. The same phenomenon was observed mouse Using lineage-negative Sca-1 c-KIT PSGL-1/ wild-type mice, we establish PSGL-1 ligand involved growth inhibition apoptosis. Moreover, stable transfection myeloid cell line K562 (which express PSGL-1) with (1,3) fucosyltransferase VII alone sufficient recapitulate Eselectin–mediated cells. These data an ligand(s) other than transduces inhibitory proapoptotic signals requires posttranslational fucosylation be functional. (Blood. 2004;103: 1685-1692)