作者: Seiya Takahashi , Satoshi Akagi , Kazutsugu Matsukawa
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摘要: Nuclear transfer is a complex multistep procedure that includes oocyte maturation, cell cycle synchronization of donor cells, enucleation, fusion, activation and embryo culture. Therefore, many factors are believed to contribute the success development following nuclear transfer. Numerous attempts improve cloning efficiency have been conducted since birth first sheep by somatic However, has remained low, applications limited. In this review, we discuss some affect developmental