Characterization of the human atheroma plaque secretome by proteomic analysis.

摘要: Atherosclerosis is one of the most common causes death in developed countries. an inflammatory process that results development complex lesions or plaques protrude into arterial lumen. Plaque rupture and thrombosis result acute clinical complications myocardial infarction stroke. Although certain risk factors (dyslipidemias, diabetes, hypertension) humoral markers plaque vulnerability (C-reactive protein, interleukin-6, -10 -18, CD-40L) have been identified, a highly sensitive specific biomarker protein profile, which could provide information on stability/vulnerability atherosclerotic lesions, remains to be identified. Recently, we described novel strategy consisting proteomic analysis proteins released by normal walls culture. This method enables harvesting are only secreted pathological walls. By focusing found tissue culture media, there intended bias toward those molecules would higher probability later being plasma. Using this approach, shown carotid cultured vitro able secrete proteins, also differential pattern secretion arteries vs ones has observed. In chapter, human atheroma secretome described.