Widespread plasticity in CTCF occupancy linked to DNA methylation
作者： H. Wang , M. T. Maurano , H. Qu , K. E. Varley , J. Gertz
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摘要: CTCF is a ubiquitously expressed regulator of fundamental genomic processes including transcription, intra- and interchromosomal interactions, chromatin structure. Because its critical role in genome function, binding patterns have long been assumed to be largely invariant across different cellular environments. Here we analyze genome-wide occupancy by ChIP-seq 19 diverse human cell types, normal primary cells immortal lines. We observed highly reproducible yet surprisingly plastic landscapes, indicative strong cell-selective regulation occupancy. Comparison with massively parallel bisulfite sequencing data indicates that 41% variable linked differential DNA methylation, concentrated at two positions within the recognition sequence. Unexpectedly, were markedly versus cells, latter showing widespread disruption associated increased methylation. Strikingly, this accompanied up-regulation expression, result both maintain same average number sites genome-wide. These results reveal tight linkage between methylation global major sequence-specific regulatory factor.
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