The 60 S ribosomal subunit as a carrier of eukaryotic initiation factor 2 and the site of reversing factor activity during protein synthesis.
作者： N S Thomas , R L Matts , D H Levin , I M London
DOI: 10.1016/S0021-9258(17)39316-X
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摘要: Studies on the recycling of eukaryotic initiation factor 2 (eIF-2) during protein synthesis in normal and heme-deficient reticulocyte lysates indicate that eIF-2 binds physiologically to 60 S ribosomal subunit. Several findings suggest subunit serves as a carrier for synthesis. The addition purified (beta-32P) hemin-supplemented results its binding polyribosomal subunits; is temperature-dependent. In inhibited by heme deficiency, phosphorylated alpha can be detected subunits early initial linear phase synthesis; after disaggregation shut-off synthesis, accumulates free ribosome 80 couples. associated with appears present binary complex [eIF-2 (alpha P) X GDP]; this tight not affected treatment 25 mM EDTA or sedimentation sucrose gradients. Reversal inhibition reversing rapid concomitant dissociation [eIF-2(alpha GDP]. These GDP] formed assembly polyribosomes subsequently released action factor.
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