Electrophysiological and molecular analysis of aminergic neurons controlling arousal

摘要: One of the most important roles histaminergic and dopaminergic systems is regulation sleep-waking cycle (Brown et al., 2001). In my thesis I colleagues showed effect several well-known wake (or sleep) - promoting neurotransmitters substance on activity HAergic DAergic neurons. Adenosine does not change firing rate or membrane potential TMN neurons in hypothalamic brain slices (Sergeeva 2006). These results support idea about indirect modulation with adenosine. contrast, ATP, ADP UTP excite With a help expression analysis we found that P2Y1 P2Y4 receptors are major target for purines neurons. The other point this work interactions between glial cells Our immunostaining revealed metabotropic widely expressed both nuclear cells. Moreover, ATP was reduced but eliminated completely by prior application antagonist. According to data proposed activated after uptake nucleotides followed increase glutamate release 2006). TRH increased induced depolarization directly through TRH1 TRH2 (Parmentier preparation). On hand, mediates frequency amplitude spontaneous inhibitory postsynaptic currents indicating preparation). Modafinil novel wake-promoting drug mechanism it action remains unclear. our modafinil inhibit from substantia nigra ventral tegmental area. This involve adrenergic system, related D2-like receptor activation. Considering data, suggest if unique All findings relevant better understanding role adenosine, as well regulation.