Sensitive amperometric detection of riboflavin with a whole-cell electrochemical sensor
作者： Yang-Yang Yu , Jing-Xian Wang , Rong-Wei Si , Yuan Yang , Chun-Lian Zhang
DOI: 10.1016/J.ACA.2017.06.053
关键词:
摘要: A novel whole-cell electrochemical sensor was developed and applied for sensitive amperometric detection of riboflavin. In this work, a based riboflavin redox cycling system characterized, in which electroactive bacteria Shewanella oneidensis MR-1 employed as the biocatalyst to regenerate reduced after electrode oxidation. This efficiently enhanced response enabled stable current output at poised potential. Thus, biosensing by integrating with conventional sensor. Remarkably, biosensor exhibited high sensitivity (LOD = 0.85 ± 0.09 nM, S/N = 3), excellent selectivity stability. Additionally, reliable analysis real samples (food pharmaceutical samples) achieved. work provided practical tool detection, demonstrated that integration using its outwards electron transfer would be powerful promising strategy improve performance sensing system.
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