Kinetic Modelling and Test-Retest Reproducibility for the Dopamine D1R Radioligand [11C]SCH23390 in Healthy and Diseased Mice.

摘要: Our aim in this study was to compare different non-invasive pharmacokinetic models and assess test–retest reproducibility of the radioligand [11C]SCH23390 for quantification dopamine D1-like receptor (D1R) both wild-type (WT) mice heterozygous (HET) Q175DN as Huntington’s disease (HD) model. Adult WT (n = 9) HET (n = 14) underwent a 90-min positron emission tomography (PET) scan followed by computed (CT) evaluate modelling healthy diseased conditions. Additionally, 5 7 animals received second PET reproducibility. Parallel assessment simplified reference tissue model (SRTM), multilinear (MRTM) Logan (Logan Ref) using striatum receptor-rich region cerebellum receptor-free (reference) performed define most suitable method regional- voxel-based binding potential (BPND). Finally, standardised uptake value ratio (SUVR-1) assessed measurement. For all models, we measured significant decline D1R density (e.g. SRTM = − 38.5 ± 5.0 %, p < 0.0001) compared littermates. Shortening duration resulted large underestimation striatal BPND (SRTM 60 min: − 17.7 ± 2.8 %, p = 0.0078) − 13.1 ± 4.1 %, p = 0.0001). Striatal measurements were very reproducible with an average variability below 5 % when MRTM SRTM. Parametric maps generated SRTM highly reliable, showing nearly perfect agreement regional analysis (r2 = 0.99, p < 0.0001). provided accurate estimate relative tracer delivery R1 analyses. SUVR-1 at time intervals not sufficiently reliable (r2 < 0.66). Ninety-minute acquisition use is recommended. imaging demonstrates optimal characteristics psychiatric neurological disorders exemplified mouse HD.