Amniotic mesenchymal tissue cells inhibit dendritic cell differentiation of peripheral blood and amnion resident monocytes.

摘要: Cells derived from the amniotic membranes of human term placenta have drawn much interest for their characteristics multipotency and low immunogenicity, supporting a variety possible clinical applications in field cell transplantation regenerative medicine. We previously shown that cells mesenchymal region amnion (AMTC) can strongly inhibit T-lymphocyte proliferation. In this study, we demonstrate AMTC block differentiation maturation monocytes into dendritic (DC), preventing expression DC marker CD1a reducing HLA-DR, CD80, CD83. The monocyte resulted impaired allostimulatory ability these on allogeneic T cells. attempting to define mechanisms responsible findings, observed presence differentiating cultures results arrest G(0) phase abolishes production inflammatory cytokines such as TNF-alpha, CXCL10, CXCL9, CCL5. Finally, also monocytic present fail differentiate toward lineage. Taken together, our data suggest by which exert immumodulatory effects do not only relate directly cells, but include inhibition generation antigen-presenting context, represent very attractive source multipotent promise be remarkably valuable approaches, due because added potential modulating immune responses, could fundamental both controlling graft rejection after diseases characterized processes.