Opposite Effects of Lysophosphatidylethanolamines on Conformation of OmpF-like Porin from Yersinia pseudotuberculosis.

摘要: Lysophosphatidyletnolamine (LPE) is one of enigmatic lipids bacteria. It generated from major membrane lipid - phosphatidylethanolamine at severe changes the bacterial growth conditions. Accumulation this phospholipid in cells Gram-negative enterobacterium Yersinia pseudotuberculosis results enhanced thermostability OmpF-like porin (YOmpF) same The respective integral conformational rearrangements may disturb channel permeability protein under stress However, role fatty acid composition LPE effect remained unclear. Present work demonstrated that level unsaturated 3.5 times higher than saturated total exposed to (phenol treatment). Unsaturated 1-oleoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine (MOPE) and 1-palmitoyl-2- hydroxy-sn-glycero-3-phosphoethanolamine (MPPE) oppositely affect conformation YOmpF. MOPE increases thermal stability due more dense packing monomers preserves its trimeric form elevated temperature, while MPPE weakens contact between promotes dissociation protein.