Myeloid calcium binding proteins: expression in the differentiated HL-60 cells and detection in sera of patients with connective tissue diseases.

摘要: A differentiation antigen induced in 1,25-dihydroxyvitamin D3 (VD3)-treated HL-60 cells was identified as being comprised of the myeloid calcium binding proteins CaBP-p8 and -p14 by determining its amino acid DNA sequence. Northern blot analysis using a fragment gene encoding p14 probe indicated that not expressed undifferentiated but transcribed starting on day 1 after VD3 treatment. The level p 14 mRNA reached peak 2, then declined, little remained 10, indicating is activated once inactivated during due to VD3. In contrast, thymidylate synthase (TSase) present disappeared quickly Both p8 CaBP were found at elevated levels sera some patients with connective tissue diseases [highly rheumatoid arthritis (RA), Sjogren's syndrome (SjS), systemic lupus erythematosus (SLE), progressive sclerosis (PSS), moderately polymyositis or dermatomyositis (PM/DM) mixed disease (MCTD)]. These results sharp contrast finding always highly cystic fibrosis (CF) [Bruggen et al. (1988) Nature 331, 570).