The investigation of Epstein-Barr viral sequences in 41 cases of Burkitt's lymphoma from Egypt: epidemiologic correlations.

摘要: Background. Epstein-Barr virus (EBV) is associated with many human neoplasms, including Burkitt's lymphoma (BL). Endemic BL in central Africa more often EBV-associated than the United States, where sero-conversion for EBV occurs somewhat later Africa. Therefore, association rate may correlate socioeconomic status of population studied, which influences age seroconversion, such factors as malaria, relate to overall higher incidence endemic regions. Methods. Forty-one patients Egypt, differs both climatically and racially from African countries (i.e., Kenya, Uganda) endemic, were analyzed. All biopsies evaluated EBV-encoded RNAs (EBER1) by RNA situ hybridization, analyzed p53 protein expression using monoclonal antibody D07, immunophenotyped a panel antibodies that included L26 (CD20), Leu 22 (CD43), A6 (CD45RO). Twelve cases evaluable subtype polymerase chain reaction EBV-specific primers. Results. The median at diagnosis was 9 years (range, 2–22 years). biopsy site extranodal 29 nodal 12 patients. 41 documented B-cell neoplasms. A hybridization signal EBER1 identified greater 95% neoplastic cells 30 (73%), whereas no observed 11 (27%). 1 found 10 patients, 2 two Immunostaining 5% 37 (24%). No significant correlation between positivity sex, site, or immunostaining. Conclusions. prevalence Egypt slightly lower regions, but significantly sporadic BL. probably reflects patient population, seroconversion. suggests immunodeficiency does not play role Egyptian lymphoma, contrast holoendemic malaria thought contribute immunodeficiency, rate, 2.