Tracking T and B cells from two-photon microscopy imaging using constrained SMC clusters.
作者： Iván Gómez-Conde , David N. Olivieri , Carlos E. Tadokoro , José Faro , José Faro
DOI: 10.2390/BIECOLL-JIB-2011-180
关键词:
摘要: Summary This paper describes a novel software algorithm, called constrained Sequential Monte Carlo (SMC) clusters, for tracking large collection of individual cells from intra-vital two-photon microscopy image sequences. We show how our method and tool, implemented in python, is useful quantifying the motility T B lymphocytes involved an immune response vs under non conditions. describe theory behind algorithm briefly discuss architecture software. Finally, we demonstrate both functionality utility by applying it to two practical examples videos displaying lymphocyte cell zones (follicles) lymph nodes.
imbio.de
uni-bielefeld.de参考文章(27)
Sequential Monte Carlo methods in practice Springer. ,(2001) , 10.1007/978-1-4757-3437-9
Joana S. Moreira, Jose Faro, Modelling two possible mechanisms for the regulation of the germinal center dynamics. Journal of Immunology. ,vol. 177, pp. 3705- 3710 ,(2006) , 10.4049/JIMMUNOL.177.6.3705
Steven H. Kleinstein, Getting Started in Computational Immunology PLoS Computational Biology. ,vol. 4, pp. e1000128- ,(2008) , 10.1371/JOURNAL.PCBI.1000128
J. G. Cyster, Chemokines and Cell Migration in Secondary Lymphoid Organs Science. ,vol. 286, pp. 2098- 2102 ,(1999) , 10.1126/SCIENCE.286.5447.2098
Alan S. Perelson, Gérard Weisbuch, Immunology for physicists Reviews of Modern Physics. ,vol. 69, pp. 1219- 1268 ,(1997) , 10.1103/REVMODPHYS.69.1219
A.J. HAND, T. SUN, D.C. BARBER, D.R. HOSE, S. MACNEIL, Automated tracking of migrating cells in phase-contrast video microscopy sequences using image registration. Journal of Microscopy. ,vol. 234, pp. 62- 79 ,(2009) , 10.1111/J.1365-2818.2009.03144.X
C. D. C. Allen, T. Okada, H. L. Tang, J. G. Cyster, Imaging of germinal center selection events during affinity maturation. Science. ,vol. 315, pp. 528- 531 ,(2007) , 10.1126/SCIENCE.1136736
Anja E. Hauser, Tobias Junt, Thorsten R. Mempel, Michael W. Sneddon, Steven H. Kleinstein, Sarah E. Henrickson, Ulrich H. von Andrian, Mark J. Shlomchik, Ann M. Haberman, Definition of Germinal-Center B Cell Migration In Vivo Reveals Predominant Intrazonal Circulation Patterns Immunity. ,vol. 26, pp. 655- 667 ,(2007) , 10.1016/J.IMMUNI.2007.04.008
Tanja A. Schwickert, Randall L. Lindquist, Guy Shakhar, Geulah Livshits, Dimitris Skokos, Marie H. Kosco-Vilbois, Michael L. Dustin, Michel C. Nussenzweig, In vivo imaging of germinal centres reveals a dynamic open structure Nature. ,vol. 446, pp. 83- 87 ,(2007) , 10.1038/NATURE05573
Thorsten R. Mempel, Sarah E. Henrickson, Ulrich H. von Andrian, T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases Nature. ,vol. 427, pp. 154- 159 ,(2004) , 10.1038/NATURE02238