MALAT1 knockdown inhibits prostate cancer progression by regulating miR-140/BIRC6 axis

摘要: Abstract Background Prostate cancer (PCa) is the second most common among men globally. Long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has been reported to be implicated in tumorigenesis and progression of PCa. However, pathogenesis MALAT1 PCa not thoroughly elaborated. Methods RT-qPCR assay was conducted measure expression MALAT1, microRNA-140 (miR-140) Baculoviral IAP repeat containing 6 (BIRC6) mRNA. Protein BIRC6 detected by western blot assay. Cell proliferative ability assessed MTS Edu retention assays. migratory invasive abilities were evaluated wound healing Transwell invasion assay, respectively. apoptotic rate examined using a flow cytometry. The interaction between miR-140 or 3′UTR explored luciferase, immunoprecipitation (RIP) pull down Xenograft models established further explore role molecular mechanism MALAT vivo. Results highly expressed human tumor tissues cell lines. knockdown inhibited proliferation, migration induced apoptosis MiR-140 could directly bind with 3′UTR. Moreover, BIRC mRNA protein through upregulating cells. Additionally, xenograft growth regulating miR-140/BIRC6 axis Conclusion hindered vitro vivo, hinting potential value management