Evidence for a role of oxygen derived free radicals in the pathogenesis of caerulein induced acute pancreatitis in rats.

摘要: The effects of a polyethylene glycol linked oxygen free radical scavenger enzyme, superoxide dismutase (PEG:SOD) on caerulein induced acute pancreatitis (AP) in rats were examined. Pancreas weights and serum amylase concentrations given three hour continuous intravenous infusion (7.5 micrograms/kg/h, n = 18) for induction AP followed by normal saline significantly raised approximately 25% (p less than 0.005) 750% 0.001), respectively, compared with values obtained control (n 7) infused six hours alone. A single intraperitoneal injection either 1 X 10(4) U/kg 6), 2 5), or 4 5) PEG:SOD immediately before did not alter pancreas weights, content, pancreatic histopathology By contrast, bolus 9) treatment reduced content 0.05) U/kg/h produced significant reductions 35% 0.05), 50% 0.01) concentrations, acinar cell vacuolisation 0.01), In studies using bovine albumin to at protein identical high dose no beneficial against observed. These data suggest that (a) derived radicals are involved the early pathogenesis rats, (b) greatly extended circulating half life ( > 35 minutes native dismutase) may make this compound more suitable as potential therapeutic agent AP.