Direct comparison of current cell-based and cell-free approaches towards the repair of craniofacial bone defects - A preclinical study.

摘要: Abstract For craniofacial bone defect repair, several alternatives to graft (BG) exist, including the combination of biphasic calcium phosphate (BCP) biomaterials with total marrow (TBM) and marrow-derived mesenchymal stromal cells (MSCs), or use growth factors like recombinant human morphogenic protein-2 (RhBMP-2) various scaffolds. Therefore, clinicians might be unsure as which approach will offer their patients most benefit. Here, we aimed compare different clinically relevant tissue engineering methods in an “all-in-one” study rat calvarial defects. TBM, MSCs committed not, cultured two- three-dimensions were mixed BCP implanted bilateral parietal defects rats. RhBMP-2 BG used positive controls. After 7 weeks, significant de novo formation was observed rhBMP-2 groups, a lesser amount, when TBM three-dimensions. Due efficacy safety TBM/BCP approach, recommend this one-step procedure for further clinical investigation. Statement Significance (BM) BM stem cell (MSC)-based regenerative medicine have shown promising alternative grafting (BG). not ceramics (CaP) CaP MSCs. Since BM-based does require harvest culture, but provides formation, consideration strategy applications.