Deciphering the Epigenetic Code of Cardiac Myocyte Transcription

摘要: Rationale: Epigenetic mechanisms are crucial for cell identity and transcriptional control. The heart consists of different types including cardiac myocytes, endothelial cells, fibroblasts others. Therefore, type-specific analysis is needed to gain mechanistic insight into the regulation gene expression in myocytes. While cytosolic mRNA represents steady-state levels, nuclear more closely reflects activity. To unravel epigenetic control, modifications crucial. Objective: aim was purify myocyte nuclei from hearts species by magnetic- or fluorescent-assisted sorting determine cellular RNA profiles marks a myocyte-specific manner. Methods Results: Frozen tissue samples were used isolate nuclei. High purity confirmed isolated mice, rats humans. Deep sequencing revealed major fraction nascent, unspliced contrast results obtained purified Cardiac showed differences especially metabolic genes. Genome-wide maps elongation mark H3K36me3 generated chromatin-immunoprecipitation. Transcriptome data high degree myocyte-specificity our protocol. An integrative histone occurrence indicated impact chromatin state on activity myocytes. Conclusions: This study establishes as universal method investigate processes myocytes origins. These sets provide novel transcription.