Optimization of exopolysaccharide production from Pseudomonas stutzeri AS22 and examination of its metal-binding abilities.

摘要: Aims To investigate the effect of culture conditions and medium components on exopolysaccharide (EPS) production by Pseudomonas stutzeri AS22 to access EPS performance as a metal-binding exopolysaccharide. Methods Results The Ps. stutzeri in submerged were optimized using two approaches for quantification, its capacity was evaluated both single mixed metal ions systems. Maximum level achieved after 24 h incubation at 30°C with an initial pH 8·0, 250 rev min−1 stirring 10% inoculum size. 50 g l−1 starch, 5 g l−1 yeast extract, 0·5 g l−1 NaCl, 1·4 g l−1 K2HPO4, 0·4 g l−1 MgSO4, CaCl2 1 g l−1 mannose found be most suitable carbon, nitrogen, mineral additional carbohydrate sources, respectively. From experiments, crude showed interesting adsorption adopting order Pb >> Co > Fe > Cu >> Cd. Lead preferentially biosorbed maximal uptake 460 mg g−1 EPS. Conclusions Under optimal requirements, reached 10·2 g l−1 fermentation, seven times more than under conditions. According assay, has potential used novel biosorbent treatment heavy metals-contaminated water. Significance Impact Study Our results are terms yield well efficiency use metal-absorbent polymer bioremediation field.