Combination of lentivirus-mediated silencing of PPM1D and temozolomide chemotherapy eradicates malignant glioma through cell apoptosis and cell cycle arrest

摘要: Temozolomide (TMZ) is approved for use as first-line treatment glioblastoma multiforme (GBM). However, GBM shows chemoresistance shortly after the initiation of treatment. In order to detect whether silencing human protein phosphatase 1D magnesium dependent (PPM1D) gene could increase effects TMZ in glioma cells, cells U87-MG were infected with lentiviral shRNA vector targeting PPM1D silencing. After was established, treated TMZ. The multiple functions and chemotherapy detected by flow cytometry MTT assay. Significantly differentially expressed genes distinguished microarray-based expression profiling analyzed pathway enrichment analysis ontology assessment. Western blotting used establish core genes. improves induced cell proliferation induces apoptosis cycle arrest. When combined performed 367 upregulated 444 downregulated compared negative control. most significant differential cancer IGFR1R, PIK3R1, MAPK8 EP300 are network. showed that PIK3R1 levels RB1 decreased. It consistent profiling. conclusion, eradicates through PIK3R1/AKT plays a role chemotherapy.