作者: S. Kearsey
DOI: 10.1002/J.1460-2075.1983.TB01626.X
关键词:
摘要: A method is presented for rapid sequencing and mapping of elements which support autonomous replication in yeast. The strategy relies on a novel phage M13 vector allows detection ARS (autonomously replicating sequence) function cloned fragments. Deletion an element linked to the HO gene Saccharomyces cerevisiae has identified 57-bp region 3' gene, essential replication. This shows sequence homology other elements.