Discovery of posttranscriptional regulatory RNAs using next generation sequencing technologies.

摘要: Next generation sequencing (NGS) has revolutionized the way by which we engineer metabolism radically altering path to genome-wide inquiries. This is due fact that NGS approaches offer several powerful advantages over traditional methods include ability fully sequence hundreds thousands of genes in a single experiment and simultaneously detect homozygous heterozygous deletions, alterations gene copy number, insertions, translocations, exome-wide substitutions "hot-spot mutations." chapter describes use these technologies as technique for transcriptome analysis discovery regulatory RNA elements context three main platforms: Illumina HiSeq, 454 pyrosequencing, SOLiD sequencing. Specifically, this focuses on since it most widely used platform analysis. Regulatory RNAs have now been found all branches life. In bacteria, noncoding small (sRNAs) are involved highly sophisticated circuits quorum sensing, carbon metabolism, stress responses, virulence (Gorke Vogel, Gene Dev 22:2914-2925, 2008; Gottesman, Trends Genet 21:399-404, 2005; Romby et al., Curr Opin Microbiol 9:229-236, 2006). Further characterization underlying regulation expression remains poorly understood given estimated 60% predicted remain hypothetical 5' 3' untranslated regions unknown more than 90% (Siegel Parasitol 27:434-441, 2011). Importantly, manipulation posttranscriptional occurs at level stability export, trans-splicing, polyadenylation, protein translation, via (Clayton, EMBO J 21:1881-1888, 2002; Haile Papadopoulou, 10:569-577, 2007) could be beneficial metabolic engineering.