Study on the inhibition of telomerase activity and regulated mechanism in human cancer cell by Icarrin

摘要: Objective:To study inhibited tecomerase activity of HL-60 cells in human cancer cell by Icarrin(ICA) and its mechanism.Methods:MTT assay,NBT assay, TRAP-PCR,RT-PCR assay,Flow Cytometry.Results:ICA telomerase significantly.It was negative correlation between the expression ratio CD11b antigen cells.It can induced to differentiate into mature granulocytes.It changed cycle distribution cells,which reflected accumulation vast majority G0/G1phase loss S phase. It downregulated proliferation related gene c-myc;upregulated p21 protein mRNA expression.Conclusion:This proved mechanism ICA on from gene-protein-effect level cells.