Significant Role of Bacterial Undecaprenyl Diphosphate (C55-UPP) for Rubber Synthesis byHevea Latex Enzymes

摘要: Washed bottom fraction (BF) membrane-bound particles of centrifuged fresh Hevea latex were found to be very active in rubber biosynthesis (RB). The washed BF membrane (WBM) showed higher RB activity and is strongly stimulated by anionic surfactants - more DOC than SDS. WBM enzymes system can synthesize either with allylic isoprenes (higher RB) or without (lower RB). (WRP), used generally assays, had low compared the much observed for WBM. Bacterial undecaprenyl diphospate (C 55 -UPP) was initiator synthesis Comparisons UPP shorter ones 15 -FPP, C 20 -GGPP) that most effective. orders >> GGPP > FPP. activated synthesized less polyprenyl intermediates (butanol extractable) but final product (toluene/hexaue extract), different FPP GGPP. highly versatile using diverse allylics, preferable. WRP a little DOC, still lower Rubber analysis RP-TLC acetone/ hexane solvent mostly rubber, mainly intermediates. Quantitative labeled confined origin spot, as It thus confirmed plays key role functions, not reported. served actual site, bacterial good initiator.