Near-infrared excited cooperative upconversion in luminescent Ytterbium(ΙΙΙ) bioprobes as light-responsive theranostic agents.

摘要: Abstract Near-infrared (NIR) Ytterbium(ΙΙΙ) complexes namely [Yb(dpq)(DMF)2Cl3] (1), [Yb(dppz)(DMF)2Cl3] (2), [Yb(dpq)(ttfa)3] (3) and [Yb(dppz)(ttfa)3] (4) based on photosensitizing antenna: dipyrido-[3,2-d:2′,3′-f]-quinoxaline (dpq), dipyrido-[3,2-a:2′,3′-c]-phenazine (dppz) 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione (Httfa), were designed as NIR bioimaging agents utilizing cooperative upconversion luminescence (CUCL) of Yb(III). Their structures, detailed photophysical properties, biological interactions, photo-induced DNA cleavage, photocytotoxicity cellular internalization properties examined. Discrete mononuclear adopt a seven-coordinated {LnN2O2Cl3} mono-capped octahedron (1, 2) eight-coordinated {LnN2O6} distorted square antiprism geometry (3, 4) with bidentate N, N-donor dpq, dppz O,O-donor ttfa ligands. The Yb(III) probes having advantages dual sensitizing antennae (dpq/dppz Httfa) to modulate the desirable optical in region for biologically transparent window light-responsive intracellular damage spatiotemporal control. lack inner-sphere water (q = 0), remarkable photostability, large Stokes' shifts, presence energetically rightly poised ligand 3T states allows efficient energy transfer (ET) emissive 2F5/2 state Yb(ΙΙΙ). unique was observed 1–4 visible blue (λem = 490 nm) upon excitation at 980 nm, makes them special candidates NIR-to-visible or NIR-to-NIR imaging probes. CUCL property discrete both solid solution. We elegantly utilized this application first time our knowledge including potential uses CUC/multiphoton microscopy. exhibit significant binding propensity DNA, HSA BSA (K ∼ 105 M−1). They effectively cleave supercoiled (SC) its nicked circular (NC) form 365 nm via photoredox pathways. uptake studies evidently displayed cytosolic nuclear localization complexes. Finally, capability usage PDT demonstrated through near-IR 980 nm CW laser. results depicted here offers an intelligent strategy towards developing highly photostable theranostic phototherapeutic window.