From Accumulation to Degradation: Reprogramming Polyamine Metabolism Facilitates Dark-Induced Senescence in Barley Leaf Cells.

摘要: The aim of this study was to analyze whether polyamine (PA) metabolism is involved in dark-induced Hordeum vulgare L. ‘Nagrad’ leaf senescence. In the cell, titer PAs relatively constant and carefully controlled. Senescence-dependent increases free putrescine, spermidine spermine occurred when process induced, accompanied by formation putrescine conjugates. addition anti-senescing agent cytokinin, which delays senescence, dark-incubated leaves slowed senescence-dependent PA accumulation. A feature senescence initial accumulation at beginning their subsequent decrease during later stages. Indeed, both enhanced expression biosynthesis catabolism genes an increase activity enzymes two metabolic pathways. To confirm capacity plant control might be linked PA, chlorophyll fluorescence parameters, nitrogen status senescing barley were measured after inhibition exogenously applied γ-aminobutyric acid (GABA). results obtained blocking oxidation showed that accelerated. However, inhibitor together with GABA, continued without disruption. On other hand, delayed process. It could concluded leads facilitating catabolism. Putrescine supports through GABA production spermidine/spermine degradation processes, as verified H2O2 production.