Whole Genome Sequencing and Characterization of Multidrug-Resistant (MDR) Bacterial Strains Isolated From a Norwegian University Campus Pond.

摘要: The presence of extended-spectrum β-lactamase (ESBL)-producing bacteria in environmental sources has been reported worldwide and constitutes a serious risk community-acquired infections with limited treatment options. current study aimed to explore the these worrisome pond located at Norwegian University Life Sciences As, Norway. A total 98 bacterial isolates survived growth on selective chromogenic media were identified by 16S rRNA Sanger sequencing. All strains evaluated for most commonly found β-lactamases ESBLs clinical settings (blaCTX-M groups 1, 2 9, blaCMY, blaSHV, blaTEM) carbapenemases (blaIMP, blaKPC, blaNDM, blaOXA, blaSFC1, blaVIM) through multiplex PCR. eight determined contain one or more genes interest. Phenotypic resistance 18 antimicrobial agents was assessed subjected whole genome sequencing combination Oxford Nanopore’s MinION Illumina’s MiSeq. Results revealed ESBL-producing Escherichia coli, Klebsiella pneumoniae, Stenotrophomonas maltophilia Paraburkholderia spp. Identified include blaCTX-M, blaTEM, blaSHV possible blaKPC-like gene, both documented novel sequences established. In addition, two inducible beta-lactamases found, class beta-lactamase (L1) cephalosporinase (L2). be multidrug resistant numerous non-β-lactams observed. conclusion, this demonstrates that are potential reservoir clinically relevant may pose health humans upon exposure.