Transcription of defense related genes in Pacific white shrimp, Litopenaeus vannamei , kept in biofloc and in clear seawater and challenged with the white spot syndrome virus

摘要: Shrimp farming in biofloc technology system (BFT) has been considered a sustainable alternative to conventional production, reducing environmental impacts due zero water exchange. Besides increasing productivity, as microorganisms represent additional feed for shrimp, BTF may prevent diseases. White spot disease is the most devastating viral of farmed causing large socio-economic impacts. Our aim was compare survival rates and transcription profiles target genes different tissues juvenile specimens Pacific white Litopenaeus vannamei, reared either BFT or clear seawater (CSW), after challenging with syndrome virus (WSSV). rate higher among animals kept CSW, which also presented lower load 48 h post-infection (p.i.). We could address possible individual patterns sole survivor at 72 h p.i. biofloc, probably less vulnerable shrimp WSSV. There no significant difference regarding profile between systems before WSSV infection; gene apparently modulated by infection. Changes were more frequently observed hepatopancreas. Out eleven selected genes, those coding Calreticulin, β-tubulin, Prophenoloxidase showed upregulation, followed QM, SOD, Ubiquitin. Although biosecure, this are not immune disease, especially against highly virulent pathogens, such