The regulation of phospholipase D by inositol phospholipids and small GTPases

摘要: Phospholipase D1 and D2 (PLD1, PLD2) both have PX PH domains in their N-terminal regions with these inositol lipid binding playing key roles regulating PLD activity localisation. The of PLD1 is also regulated by protein kinase C members the Rho Arf families GTPases. Each proteins binds to unique sites; however, there appears be little vitro discrimination between individual family members. In agonist-stimulated cells, specificity, with, for example RBL-2H3 antigen stimulating activation association Arf6, Rac1 Cα. PLD2 less directly GTPases rather primarily controlled through interaction phosphatidylinositol 4-phosphate 5-kinase that generates activating 4,5-bisphosphate.