Detection of human cytomegalovirus in plasma of AIDS patients during acute visceral disease by DNA amplification.

摘要: By using the polymerase chain reaction (PCR) amplification procedure, 19 (83%) of 23 plasma specimens obtained from individuals with AIDS and human cytomegalovirus (HCMV) visceral disease were found to be positive for viremia as detected by PCR (PV-PCR), whereas 78% cultures peripheral blood leukocytes same samples positive. All 11 prospectively acute HCMV PV-PCR. Plasma patients who received ganciclovir therapy rapidly became both culture PV-PCR negative, there was an excellent correlation between two procedures. DNA unaffected filtering through a 0.2-microns-pore-size filter, although conserved cellular gene, HLA-DQ alpha, undetectable following filtration. in could quantitated endpoint serial dilutions, detectable virus being present 10(1) 10(-2) microliters plasma. A low titer infectious 2 samples. The detection promises useful procedure monitoring suspected having impending, acute, or recurrent suggests additional route which may disseminate immunocompromised host. Images