Low-serum culture system improves the adipogenic ability of visceral adipose tissue-derived stromal cells.
作者： Hiroshi Nagasaki , QingLong Shang , Takeshi Suzuki , Hiroyuki Hashimoto , Tomoko Yoshimura
DOI: 10.1042/CBI20100406
关键词:
摘要: In obese adipose tissue, infiltrating macrophages release proinflammatory cytokines that trigger insulin resistance. An adipocyte-based platform from visceral fat would be useful to elucidate the pathology of inflammation and develop therapeutic drugs for ADSCs (adipose tissue-derived mesenchymal stromal cells) expanded subcutaneous are intensively studied as sources regenerative medicine. However, adipocyte culture system tissue has not been utilized yet. We aimed establish bioactive using pad. Stromal vascular fractions were processed epididymal pads Sprague-Dawley rats three human omental pads, a low-serum method. The responses ADSC-adipocytes (their adipogenic lineages) pioglitazone, drug diabesity, evaluated by gene expression ELISA. (1×108) 10 g rat or over five passages. Cell surface marker expressions revealed equivalent stem cells. in significantly showed higher markers [PPAR (peroxisome proliferator-activated receptor) γ, LPL (lipoprotein lipase) FABP4 (fatty acid-binding protein 4)] adipocytokines [adiponectin, resistin, leptin, PAI-1 (plasminogen-activator inhibitor 1) IL (interleukin)-10] than those high-serum system. Pioglitazone accelerated induction increased adiponectin 57.9±5.8-fold (mean±S.E.M.) relative control cells (P<0.001). Both ADSC-adipocytes, TNF-α induced [MCP-1 (monocyte chemoattractant protein-1) IL-6] suppressed expression, while pioglitazone antagonized these effects. present findings suggest adiposcience pharmacological evaluations.
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