Antigen presentation by human dermal fibroblasts: activation of resting T lymphocytes.

摘要: We have shown that human dermal fibroblasts, exposed to interferon-gamma (IFN-gamma) induce surface class II major histocompatibility complex (MHC) antigens, were capable of presenting tetanus toxoid (TT) antigen TT-specific T cell clones. Antigen presentation by fibroblasts was dependent, required HLA-DR expression and MHC restricted. In contrast, we now report IFN-gamma-treated are unable present TT purified resting cells obtained from the peripheral blood TT-immune donors. addition, although able stimulate alloreactive clones, they themselves primary allogeneic responses in cells. The failure not due suppressor effects because induction alloantigen monocytes inhibited presence fibroblasts. On contrary, IFN-treated synergistic with small numbers activating reversed addition recombinant interleukin 2 (rIL 2) cultures, but 1 (IL 1). These results emphasize requirements for activation differ those Although can efficiently requires exogenous IL 2. It is postulated classical antigen-presenting incapable stimulating production