The proteomics of sickle cell disease: profiling of erythrocyte membrane proteins by 2D-DIGE and tandem mass spectrometry.
作者： David G. Kakhniashvili , Natalya B. Griko , Lee A. Bulla , Steven R. Goodman
DOI: 10.1177/153537020523001102
关键词:
摘要: Quantitative changes in the red blood cell membrane proteome sickle disease were analyzed using two-dimensional fluorescence difference gel electrophoresis 2D-DIGE technique. From over 500 spots, we found 49 protein spots whose content membranes changed by at least 2.5-fold as compared to control cells. In 38 cases observed an increase and 11 a decrease membranes. The proteins of interest identified in-gel tryptic digestion followed liquid chromatography line with tandem mass spectrometry. 44 forms representing different modifications 22 original sequences. majority fall into small groups related following five categories: actin accessory proteins--four proteins, components lipid rafts--two scavengers oxygen radicals--two repair participants--six turnover components--three proteins. number RBC is decreased noticeably smaller, most are either rafts or Elevated participants well radical may reflect increased oxidative stress
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