Isolation of BAC clones containing conserved genes from libraries of three distantly related moths: a useful resource for comparative genomics of Lepidoptera.
作者： Yuji Yasukochi , Makiko Tanaka-Okuyama , Manabu Kamimura , Ryo Nakano , Yota Naito
DOI: 10.1155/2011/165894
关键词:
摘要: Lepidoptera, butterflies and moths, is the second largest animal order includes numerous agricultural pests. To facilitate comparative genomics in we isolated BAC clones containing conserved putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, Plutella xylostella, harboring haploid chromosome number, n = 31, which are not closely related with each other or silkworm, Bombyx mori, (n 28), sequenced model lepidopteran. A total 108–184 representing 101–182 were for species. For 79 genes, more than two species, will be useful as common markers analysis using fluorescence situ hybridization (FISH), well comparison genome sequence among multiple The PCR-based clone isolation method presented here applicable to species lack a but have significant collection cDNA EST sequences.
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