Transparent and Robust Silica Coatings with Dual Range Porosity for Enzyme‐Based Optical Biosensing

摘要: Hierarchically porous transparent silica coatings combine large specific surface area with enhanced pore accessibility for optical biosensing. This paper describes a versatile approach to fabricate optically multiscale porosity. Thin films (around 1 mu m in thickness) of an aqueous suspension primary aggregates form mesoporous, interconnected matrix, and sacrificial polymer particles template well-defined, discrete macropores high structural integrity. The total achieved is around 200 m(2) g(-1) mesopore sizes 20-40 nm 250 nm, porosity 84%. macro/meso dual range allows biocatalyst loadings l-lactate dehydrogenase detection lactate. functionalized showed linear response within the interest 1-20 x 10(-3) These biosensing therefore strongly enhance sensitivity, speed reliability based lactate as compared classical thin monomodal structure. Particle-based simulations experiments reveal that both location connectivity control performance. procedure presented here are versatile, scalable, inexpensive, compatible wide deposition techniques suitable industrial health care applications.