INN-toxin, a highly lethal peptide from the venom of Indian cobra (Naja naja) venom-Isolation, characterization and pharmacological actions.
作者： K.C. Ponnappa , Pushpa Saviour , N.B. Ramachandra , R. Manjunatha Kini , T. Veerabasappa Gowda
DOI: 10.1016/J.PEPTIDES.2008.07.020
关键词:
摘要: Abstract A novel toxic polypeptide, INN-toxin, is purified from the venom of Naja naja using combination gel-permeation and ion-exchange chromatography. It has a molecular mass 6951.6 Da as determined by MALDI-TOF/MS N-terminal sequence LKXNKLVPLF. showed both neurotoxic well cytotoxic activities. INN-toxin lethal to mice with LD 50 1.2 mg/kg body weight. IgY raised in chicks against basic peptide pool neutralized toxicity INN-toxin. did not inhibit cholinesterase activity. Ehrlich ascites tumor (EAT) cells, but it leukocyte culture. The toxin appears be specific its mode action. Interaction N -bromosuccinamide (NBS) resulted modification tryptophan residues loss
elsevier.com
sci-hub.se 参考文章(31)
John King, Practical Clinical Enzymology ,(1965)
S Chwetzoff, S Tsunasawa, F Sakiyama, A Ménez, Nigexine, a Phospholipase A2 from Cobra Venom with Cytotoxic Properties Not Related to Esterase Activity: Purification, amino acid sequence, and biological properties Journal of Biological Chemistry. ,vol. 264, pp. 13289- 13297 ,(1989) , 10.1016/S0021-9258(18)51627-6
M. Ohno, R. Ménez, T. Ogawa, J.M. Danse, Y. Shimohigashi, C. Fromen, F. Ducancel, S. Zinn-justin, M.H. Le du, J.-C. Boulain, T. Tamiya, A. Ménez, Molecular Evolution of Snake Toxins: Is the Functional Diversify of Snake Toxins Associated with a Mechanism of Accelerated Evolution? Progress in Nucleic Acid Research and Molecular Biology. ,vol. 59, pp. 307- 364 ,(1997) , 10.1016/S0079-6603(08)61036-3
You-Cheng Hseu, Chien-Min Chiang, Kun-Yi Chien, A. A. Vyas, Wen-Guey Wu, G. S. Rule, Two Distinct Types of Cardiotoxin as Revealed by the Structure and Activity Relationship of Their Interaction with Zwitterionic Phospholipid Dispersions Journal of Biological Chemistry. ,vol. 269, pp. 14473- 14483 ,(1994) , 10.1016/S0021-9258(17)36647-4
George L. Ellman, K.Diane Courtney, Valentino Andres, Robert M. Featherstone, A new and rapid colorimetric determination of acetylcholinesterase activity Biochemical Pharmacology. ,vol. 7, pp. 88- 95 ,(1961) , 10.1016/0006-2952(61)90145-9
Jeffrey E. Fletcher, Ming-Shi Jiang, Possible mechanisms of action of cobra snake venom cardiotoxins and bee venom melittin Toxicon. ,vol. 31, pp. 669- 695 ,(1993) , 10.1016/0041-0101(93)90375-S
A.L. Harvey, R.J. Marshall, E. Karlsson, Effects of purified cardiotoxins from the Thailand cobra (Naja naja siamensis) on isolated skeletal and cardiac muscle preparations Toxicon. ,vol. 20, pp. 379- 396 ,(1982) , 10.1016/0041-0101(82)90001-0
K. H. Ruan, J. Spurlino, F. A. Quiocho, M. Z. Atassi, Acetylcholine receptor-alpha-bungarotoxin interactions: determination of the region-to-region contacts by peptide-peptide interactions and molecular modeling of the receptor cavity. Proceedings of the National Academy of Sciences. ,vol. 87, pp. 6156- 6160 ,(1990) , 10.1073/PNAS.87.16.6156
Chang-Shin Lee, Thallampuranam Krishnaswamy S. Kumar, Lu-Yun Lian, Jya-Wei Cheng, Chin Yu, Main-chain dynamics of cardiotoxin II from Taiwan cobra (Naja naja atra) as studied by carbon-13 NMR at natural abundance: delineation of the role of functionally important residues. Biochemistry. ,vol. 37, pp. 155- 164 ,(1998) , 10.1021/BI971979C
S.H. Chiou, C.C. Hung, H.C. Huang, S.T. Chen, K.T. Wang, C.C. Yang, Sequence Comparison and Computer Modeling of Cardiotoxins and Cobrotoxin Isolated from Taiwan Cobra Biochemical and Biophysical Research Communications. ,vol. 206, pp. 22- 32 ,(1995) , 10.1006/BBRC.1995.1004