In vivo optical imaging of membrane-type matrix metalloproteinase (MT-MMP) activity.

摘要: Herein we demonstrate for the first time that a fluorogenic probe can be used as an in vivo imaging agent visualizing activities of membrane-tethered, membrane-type matrix metalloproteinases (MT-MMPs). An MT-MMP consisted MT1-MMP (MMP-14) substrate and near-infrared (NIR) dye-quencher pair exhibited rapid, efficient boosts fluorescence upon cleavage by tumor-bearing mice. In particular, unlike similar probes designed to target extracellular, soluble-type MMPs (EC-MMPs)--which cleared from bloodstream after activation--the signals activated enable clear visualization MT1-MMP-positive tumors animal models up 24 h. The results indicate simple form is less effective EC-MMP vivo. These findings widely applied designing applications targeting various membrane-anchored proteases